• This should be the easiest part of the manuscript as it simply details what was done
  • Start writing this section during the study or research process to keep track of all steps; ask coauthors to provide details of their specific contributions
  • Do not draw conclusions or discuss results in this section; the Materials and Methods section is only for presenting what was done
  • Include IRB approval, informed patient consent statement, Animal Care and Use approval
    • Note: signed patient consent forms are not submitted with a manuscript; only a statement confirming that patient consent was obtained
  • Describe study steps chronologically: which materials/patients were selected and what was done, step-by-step
  • Be sure all methods are standard, accepted, and reproducible
  • Use clear subheadings, e.g., Patient Selection, Interventions, Animal Model, Data Analysis, etc.
  • Use generic names for medications, drugs
  • List manufacturer, city, state (country) for all lab chemicals, reagents, specific equipment, etc.; if author instructions do not state how to format manufacturer names and locations, check a recent article and follow the same format
  • Include software and version for data analysis tools