• Tell what was done chronologically: which materials/patients were selected and what was done, step-by-step
  • Be sure all methods are standard, accepted, and reproducible
  • Break into subsections with clear subheadings, i.e., Patients, Interventions, Data Analysis
  • Use generic names for medications, drugs
  • List manufacturer, city, state (country) for all lab chemicals, reagents, specific equipment, etc.
  • Include software and version for data analysis tools
  • Include IRB approval, informed consent, Animal Care and Use approval
  • Do not make comments, draw conclusions or discuss results in this section; the Materials and Methods section is only for presenting what was done